Document Details

Document Type : Thesis 
Document Title :
Physiological and Histological Studies on the Effect of Melittin on Inflammation in Mice Gastrointestinal Tract
دراسات فسيولوجية وهستولوجية حول تأثير الميليتين على التهاب القناة الهضمية في الفئران
 
Subject : biological sciences department 
Document Language : Arabic 
Abstract : Introduction: Melittin is a principle toxic peptide of bee venom. It is known as a strong anti-inflammatory agent and used as traditional medicine for treatment of different types of diseases. However, the anti-inflammatory effect of melittin on the gastrointestinal (GI) inflammation has not been elucidated. The aim of the present study is to investigate the anti-inflammatory effect of melittin on mice stomach and jejunum treated with indomethacin that induced inflammation in the GI tract of mice from the physiological, histopathological and immunohistochemical points of view. Methods: This study was performed on adult male Albino mice, which they divided into 4 groups: Control group: mice treated with distilled water; Indomethacin group treated with indomethacin (50 mg/kg) for 1day; Melittin group treated daily with melittin (10 or 40 µg/kg) for 3,5 or 10 days; Indomethacin-melittin group treated with indomethacin followed by the above melittin doses. Samples from the stomach body and jejunum collected from all animals were prepared for physiological, histopathological and immunohistochemical studies. Results: Treatment with indomethacin induced mucosal erosion and exfoliation, lamina properia and submucosal inflammation and depletion of antibodies (EMA and SMA) in the stomach and jejunum tissues. In mice treated with indomethacin, melittin reduced the histological and immunohistochemical alterations in a dose and time dependent manner. Also a significant increase of pro-inflammatory agents (5-HT and IL-1B) levels were found in the mucosa of jejunum during inflammation-induced by indomethacin, while these levels were reduced by melittin treatment. Conclusions: These data suggested that melittin can protect GI tract from the harmful effects of indomethacin. This study may help to support a potential strategy of using melittin as anti-inflammatory therapy of GI inflammation. 
Supervisor : Dr. Faiza B. Abdu 
Thesis Type : Master Thesis 
Publishing Year : 1433 AH
2012 AD 
Co-Supervisor : Prof. Osama A. Abu-Zinadah . Prof. Tarek R. Rahmy 
Added Date : Tuesday, November 27, 2012 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عبير سعيد الأحمريAl-ahmari, Abeer SaeedResearcherMaster 

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