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Document Type |
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Thesis |
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Document Title |
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Effect of Leiurus quinquestriatus Scorpion Venom on Cardiac and Skeletal Muscles in Mice تأثير سم العقرب الأصفر ليورس كوبنكيسترياتس على العضلات القلبية والهيكلية فى الفئران |
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Subject |
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biological sciences department |
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Document Language |
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Arabic |
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Abstract |
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The present study deals with the effect of the yellow scorpion Leiurus quinquestriatus venom on cardiac and skeletal muscle in mice. Animals were divided into two groups as follows: Group A: control group; and Group B: which was divided into three groups injected with a dose of (0.03 mg / kg) of scorpion venom. The first group was left for 3 hours before autopsy; the second group was left for 6 hours before autopsy and the third group was left for 9 hours before autopsy. The results of microscopic examination showed optical sectors of skeletal muscle in the control group stained with hematoxylin and Eosin, natural muscle structural terms consist of bundles of cells cylindrical his long with charts incidental Cross stations multicore Multinuclear and be the nucleus large side site, as noted on the fabric musculoskeletal under the microscope lines as a result of the presence of packs of actin filaments. Actin and myosin, which appear in the form of tablets A-disc dimmed in the microscope The drive I-disc they are not opaque, also notes the connective tissue between fiber single-supporting packages muscle (Endomysium) The connective tissue that separates packets called casing package muscle (Perimysium). After using Mason trichrome stain, the quantity of collagen fibers in muscle tissue as well as cylindrical shaped cells and muscle fibers and granules gathered inside the cytoplasm. Also, microscopic examination of the muscle structural of mice autopsied after 3 hours and stained with hematoxylin and Eosin showed laceration Disruption of simple packed muscle band, which is clear in the presence of spaces between packages, also little change in the form of nuclei as irregular arrangement in the muscle nuclei packets. On the other hand, there was adhesion of muscle packages together with adjacent fiber muscles due to a dispersion in different arrangement belts. The change in the natural shape of the muscle fibers Abnormal stricter. The cardiac control group and the muscles appear attack scheme accidental, and are made up of cells elongated and branched and are parallel to each other. The disks were observed as intercalated discs, and these structures are located in the heart muscle. The lines in muscle fibers showed Mileage longitudinally spaces, and these lines are occasional irregular interfaces. There were the nuclei of cardiac muscle fibers in the fiber center Mileage accident and shows tissue component of the pre-vacuum nucleus pronuclear space. Also, there was a free zone in the cytoplasm around the nucleus, and free muscle Alleviate. In some muscle fibers in some sectors and there are many blood vessels in the connective tissue between muscle firmness and many capillaries in muscle pillar of the package (Endomysium). After 3 hours of treatment the microscopic examination showed the muscles attack in this group therapy and a laceration evident in cardiac muscle tissue, tissue laceration and hemorrhage with blood vessels in the connective tissue between the beams of muscle with capillaries, and many in pillar packets muscle and breadth of the spaces between Weaving annexation Breadth of Interstitial connective tissue and appear disk interfaces as occasional irregular lines and occasional forest between the lines represent the ends of the contact cardiac cells. As noted Rip gravis Tissue rupture with the appearance of the nuclei of cardiac muscle fibers in fiber Mileage accidental center with a fork cardiac muscle without change in diameter. As evidenced by lack of change in the form of drive interfaces and appear in these sectors as occasional irregular lines. There are also free zones in the cytoplasm around the nucleus and Alleviate free muscle in some sectors and there are many blood vessels in the connective tissue between muscle firmness and many capillaries in muscle pillar of the package Endomysium. |
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Supervisor |
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Dr. Moustafa Hussein Roshdy Elnaggar |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1434 AH
2013 AD |
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Added Date |
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Saturday, June 15, 2013 |
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Researchers
| خلود السيف البريكي | Albriky, Kholoud Alsaif | Researcher | Master | |
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